Cellular barcoding of protozoan pathogens reveals the within-host population dynamics of

Autor: Ceire J, Wincott, Gayathri, Sritharan, Henry J, Benns, Dana, May, Carla, Gilabert-Carbajo, Monique, Bunyan, Aisling R, Fairweather, Eduardo, Alves, Ivan, Andrew, Laurence, Game, Eva-Maria, Frickel, Calvin, Tiengwe, Sarah E, Ewald, Matthew A, Child
Rok vydání: 2022
Zdroj: Cell reports methods. 2(8)
ISSN: 2667-2375
Popis: Cellular barcoding techniques are powerful tools to understand microbial pathogenesis. However, barcoding strategies have not been broadly applied to protozoan parasites, which have unique genomic structures and virulence strategies compared with viral and bacterial pathogens. Here, we present a CRISPR-based method to barcode protozoa, which we successfully apply to
Databáze: OpenAIRE