| Autor: |
Beatriz, Crego-Vicente, Pedro, Fernández-Soto, Juan, García-Bernalt Diego, Begoña, Febrer-Sendra, Antonio, Muro |
| Rok vydání: |
2022 |
| Zdroj: |
International journal of molecular sciences. 24(1) |
| ISSN: |
1422-0067 |
| Popis: |
Loop-mediated isothermal amplification (LAMP) is the most popular technology for point-of-care testing applications due its rapid, sensitive and specific detection with simple instrumentation compared to PCR-based methods. Many systems for reading the results of LAMP amplifications exist, including real-time fluorescence detection using fluorophore-labelled probes attached to oligonucleotide sequences complementary to the target nucleic acid. This methodology allows the simultaneous detection of multiple targets (multiplexing) in one LAMP assay. A method for multiplexing LAMP is the amplification by release of quenching (DARQ) technique by using a 5'-quencher modified LAMP primer annealed to 3'-fluorophore-labelled acting as detection oligonucleotide. The main application of multiplex LAMP is the rapid and accurate diagnosis of infectious diseases, allowing differentiation of co-infecting pathogens in a single reaction. Schistosomiasis, caused among other species by |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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