A radiation hybrid map of the distal short arm of human chromosome 11, containing the Beckwith-Wiedemann and associated embryonal tumor disease loci
| Autor: | C W, Richard, M, Boehnke, D J, Berg, J H, Lichy, T C, Meeker, E, Hauser, R M, Myers, D R, Cox |
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| Rok vydání: | 1993 |
| Předmět: |
Likelihood Functions
Beckwith-Wiedemann Syndrome Base Sequence Chromosomes Human Pair 11 Molecular Sequence Data Chromosome Mapping DNA Hybrid Cells Neoplasms Germ Cell and Embryonal Bacteriophage lambda Polymerase Chain Reaction Cell Line Blotting Southern Cricetinae Animals Humans DNA Probes Repetitive Sequences Nucleic Acid Research Article |
| Zdroj: | American journal of human genetics. 52(5) |
| ISSN: | 0002-9297 |
| Popis: | We describe a high-resolution radiation hybrid (RH) map of the distal short arm of human chromosome 11 containing the Beckwith-Wiedemann gene and the associated embryonal tumor disease loci. Thirteen human 11p15 genes and 17 new anonymous probes were mapped by a statistical analysis of the cosegregation of markers in 102 rodent-human radiation hybrids retaining fragments of human chromosome 11. The 17 anonymous probes were generated from lambda phage containing human 11p15.5 inserts, by using ALU-PCR. A comprehensive map of all 30 loci and a framework map of nine clusters of loci ordered at odds of 1,000:1 were constructed by a multipoint maximum-likelihood approach by using the computer program RHMAP. This RH map localizes one new gene to chromosome 11p15 (WEE1), provides more precise order information for several 11p15 genes (CTSD, H19, HPX, ST5, RNH, and SMPD1), confirms previous map orders for other 11p15 genes (CALCA, PTH, HBBC, TH, HRAS, and DRD4), and maps 17 new anonymous probes within the 11p15.5 region. This RH map should prove useful in better defining the positions of the Beckwith-Wiedemann and associated embryonal tumor disease-gene loci. |
| Databáze: | OpenAIRE |
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