Characterization of ovarian carbonyl reductase gene expression during ovulation in the gonadotropin-primed immature Rat
| Autor: | L L, Espey, S, Yoshioka, D, Russell, T, Ujioka, B, Vladu, M, Skelsey, S, Fujii, H, Okamura, J S, Richards |
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| Rok vydání: | 2000 |
| Předmět: |
Male
Ovulation Androstenols DNA Complementary Base Sequence Reverse Transcriptase Polymerase Chain Reaction Anti-Inflammatory Agents Non-Steroidal Indomethacin Molecular Sequence Data Ovary Aldo-Keto Reductases Gene Expression Regulation Developmental Blotting Northern Rats Alcohol Oxidoreductases Aldehyde Reductase Escherichia coli Animals Electrophoresis Polyacrylamide Gel Female RNA Messenger Rats Wistar Gonadotropins In Situ Hybridization Densitometry |
| Zdroj: | Biology of reproduction. 62(2) |
| ISSN: | 0006-3363 |
| Popis: | In this differential-display polymerase chain reaction-based study, four different primer sets generated cDNA fragments of ovarian carbonyl reductase genes that were uniquely expressed during the ovulatory process in eCG-primed immature rats. The temporal pattern of expression of this aldo-keto reductase gene was delineated by extracting ovarian RNA at 0, 2, 4, 8, 12, and 24 h after induction of ovulation via injection of the primed animals with hCG. The results showed that at least four homologous forms of this gene were transcribed during ovulation. Northern blot analyses indicated a 14-fold increase in ovarian mRNA for carbonyl reductase, with expression reaching a peak at 8 h after hCG treatment and then declining to negligible levels during the next 16 h. In situ hybridization revealed that most of the transcription was in the thecal connective tissue of the ovary and was absent from the granulosa layer of ovarian follicles. Treatment of the animals with ovulation-blocking doses of epostane (an inhibitor of progesterone synthesis) or indomethacin (an inhibitor of prostanoid synthesis) did not reduce the expression of ovarian carbonyl reductase. Nevertheless, the temporal pattern of expression of carbonyl reductase after the induction of ovulation suggests that this enzyme activity is at least indirectly associated with the ovulatory process. |
| Databáze: | OpenAIRE |
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