[Distribution of MIF -173 single nucleotide polymorphism checked by tetra-primer amplification refractory mutation system]
| Autor: | Bao-ying, Fei, Huo-xiang, Lu, Jian-min, Yang |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Adult
Male China Base Sequence Genotype DNA Mutational Analysis Molecular Sequence Data Middle Aged Polymerase Chain Reaction Polymorphism Single Nucleotide Intramolecular Oxidoreductases Asian People Gene Frequency Mutation Humans Female Macrophage Migration-Inhibitory Factors Alleles Polymorphism Restriction Fragment Length |
| Zdroj: | Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics. 23(3) |
| ISSN: | 1003-9406 |
| Popis: | To study the distribution of macrophagemigration inhibitory factor gene (MIF) -173 single nucleotide polymorphism (SNP) of Chinese Han population in Zhejiang province.The DNA samples were extracted from EDTA blood of 142 unrelated healthy individuals. Alleles of MIF -173 SNP were genotyped by using the techniques of tetra-primer amplification refractory mutation system (ARMS) and restriction fragment length polymorphisms (RFLP)-PCR Meantime the PCR products were cloned and sequenced.The authors detected three kinds of genotypes at the MIF -173 locus, and no deviation was observed from Hardy-Weinberg equilibrium. The final results were the same completely, whatever either tetra-primer ARMS or RFLP-PCR was used to check the MIF -173 single nucleotide polymorphism. Statistical analysis showed that the distributions of MIF -173 SNP alleles and genotype frequencies were significantly different between Chinese Han population and European Caucasian(P0.01), but no significant difference demonstrated to happen between Chinese and Japanese(P0.05).Tetra-primer ARMS is an accurate, rapid and economical method for SNP genotyping.There exists ethnic difference in the distribution of MIF -173 SNP alleles. |
| Databáze: | OpenAIRE |
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