Detection of Mycobacterium paratuberculosis in formalin-fixed paraffin-embedded tissues by the polymerase chain reaction
| Autor: | Plante, Y, Remenda, B W, Chelack, B J, Haines, D M |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1996 |
| Předmět: |
Base Sequence
Staining and Labeling Molecular Sequence Data bacterial infections and mycoses Immunohistochemistry Polymerase Chain Reaction Sensitivity and Specificity Mycobacterium avium subsp. paratuberculosis fluids and secretions parasitic diseases Paratuberculosis Animals Cattle Coloring Agents Research Article DNA Primers Retrospective Studies |
| Popis: | The polymerase chain reaction (PCR) utilizing primers specific for the IS900 sequence of Mycobacterium paratuberculosis was applied to tissue sections of formalin-fixed, paraffin-embedded ileum from cattle with Johne's disease and the results compared to those obtained with acid-fast (Ziehl-Neelsen) and immunohistochemical staining. The PCR was positive in 19/21 tissues (90%) while Ziehl-Neelsen staining was positive in 18/21 (86%) and immunohistochemical staining in 21/21 (100%). The Ziehl-Neelsen and immunohistochemical stains are not, however, specific for M. paratuberculosis. The PCR for detection of M. paratuberculosis using the IS900 sequence is a specific and relatively sensitive method for confirmation of Johne's disease and its application to formalin-fixed, paraffin-embedded tissues may be useful for confirmation of dubious cases, for retrospective studies and for epidemiological analyses. |
| Databáze: | OpenAIRE |
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