[Effects of core proteoglycan on the transdifferentiation of human renal tubular epithelial cell induced by transforming growth factor-beta1 in vitro]
| Autor: | Xue-qin, Cheng, Hua-ying, Bao, Ying, Chen, Xiao-qin, Pan, Li, Fei, Rong-hua, Chen |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Bone Morphogenetic Protein 7
Connective Tissue Growth Factor Cell Differentiation Epithelial Cells Fibroblasts Cadherins Kidney Actins Cell Line Kidney Tubules Proximal Transforming Growth Factor beta1 Kidney Tubules Transforming Growth Factor beta Cell Transdifferentiation Humans Vimentin Proteoglycans Cell Shape Cells Cultured |
| Zdroj: | Zhonghua er ke za zhi = Chinese journal of pediatrics. 45(7) |
| ISSN: | 0578-1310 |
| Popis: | To study the effects of core proteoglycan on the transdifferentiation of human renal tubular epithelial cell induced by transforming growth factor beta1 (TGF-beta1) in vitro.The cultured HK-2 cells were divided into six groups: A. negative control group; B. 10 ng/ml TGF-beta1 group; C. 10 ng/ml core proteoglycan treated group; D. 100 ng/ml core proteoglycan treated group; E. 10 ng/ml TGF-beta1 + 10 ng/ml core proteoglycan group; F. 10 ng/ml TGF-beta1 + 100 ng/ml core proteoglycan group. The changes in configuration of HK-2 cells were inspected 48 hours after adding the stimulating factor. At the same time, changes in mRNA of keratin, alpha-smooth muscle actin, vimentin were analyzed.Compared with group A, group B showed great changes in the morphology of cells, most cells converted into spindle shape, like fibroblast; groups E and F, especially group F showed significantly reduced spindle shape cells. Compared with group A, groups C and D had no significant changes in morphology of cells Compared with 10 ng/ml TGF-beta1 group and negative control, the mRNA expression of alpha-smooth muscle actin and vimentin had significant increase, but that of keratin reduced (P0.05). However, after combined treatment with TGF-beta1 and core proteoglycan, alpha-smooth muscle actin and vimentin expression were reduced significantly, while expression of keratin was up-regulated. Single core proteoglycan treated group and negative control group had no dramatic differences (P0.05).TGF-beta1 can induce the transdifferentiation of human renal tubular epithelial cell and core proteoglycan has some inhibitory effect on transdifferentiation of human renal tubular epithelial cell induced by TGF-beta1 in vitro. |
| Databáze: | OpenAIRE |
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