An Mn2+-stimulated neutral-sphingomyelinase in seminiferous tubules of immature Wistar rats

Autor:	P E, Raimann, I C, Custodio de Souza, E A, Bernard, F C, Guma
Rok vydání:	2000
Předmět:	Male Manganese Hydrolysis Age Factors Hydrogen-Ion Concentration Seminiferous Tubules Rats Sphingomyelins Sphingomyelin Phosphodiesterase Metals Microsomes Animals Magnesium Rats Wistar
Zdroj:	Molecular and cellular biochemistry. 201(1-2)
ISSN:	0300-8177
Popis:	Mammalian sphingomyelinases have been implicated in many important physiological and pathophysiological processes. The seminiferous tubules of immature (19 day-old) Wistar rats have at least three types of sphingomyelinases, a lysosomal one and two microsomal ones. One of the microsomal sphingomyelinases is active at pH 6.5 and is stimulated by Mn2+Co2+Mg2+, and the other is active at pH 7.4 and is stimulated by Mn2+Mg2+ and inhibited by Co2+. The two microsomal enzymes are only slightly inhibited by EDTA and at pH 7.4 the stimulatory effects of Mn2+ and Mg2+ are additive. These data characterize the existence of two different membrane-bound sphingomyelinases in the seminiferous tubules of the rat.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::cab324f625fdcd1d66c7ed48faa219a8 https://pubmed.ncbi.nlm.nih.gov/10630631 Zobrazit plný text záznamu Full text from SpringerLink