| Autor: |
I A, Popov, B D, Brondz, N G, Anosova, Iu S, Krivoshein, V V, Kronin |
| Rok vydání: |
1993 |
| Předmět: |
|
| Zdroj: |
Biulleten' eksperimental'noi biologii i meditsiny. 115(5) |
| ISSN: |
0365-9615 |
| Popis: |
To study T cell receptors' affinity alloantigen-specific anti-K cytotoxic T-lymphocytes (CTL) were divided on fractions by elution from donor K and third-party macrophage monolayers. The functional activity of CTL was suppressed by anti--Ly2 monoclonal antibodies (mAb), 51-Cr-labelled transformed fibroblasts (L-cells) transferred with the H-2K gene were used as targets. The results demonstrate that primary CTL enriched on third-party macrophage monolayers are the most sensitive to anti-Ly2 mAb. T-cells exhausted on third-party monolayer and then enriched on donor monolayer were resistant to treatment with the mAb. Secondary CTL enriched on donor monolayer were resistant to treatment with anti-Ly2 mAb even should they were not exhausted on third-party monolayer. These results show that Ly2 (CD8) molecule plays an essential role in the interaction of CTL with MHC class I molecule only if T-cell receptor has low affinity. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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