Role of catalytic function in the antiplatelet activity of phospholipase A2 cobra (Naja naja naja) venom

Autor:	L M, Rudrammaji, K D, Machiah, T P, Kantha, T V, Gowda
Rok vydání:	2001
Předmět:	Blood Platelets Elapid Venoms Time Factors Dose-Response Relationship Drug Epinephrine Acetophenones In Vitro Techniques Adrenergic Agonists Catalysis Phospholipases A Adenosine Diphosphate Isoenzymes Phospholipases A2 Animals Humans Collagen Enzyme Inhibitors Platelet Aggregation Inhibitors
Zdroj:	Molecular and cellular biochemistry. 219(1-2)
ISSN:	0300-8177
Popis:	Three acidic phospholipases A2 from Indian cobra (Naja naja naja) venom inhibited platelet aggregation in platelet rich plasma induced separately by ADP, collagen and epinephrine with different potencies. The order of inhibition was epinephrinecollagenADP. They did not inhibit platelet aggregation induced by arachidonic acid (10 microM). The inhibition was dependent on concentration of the protein and the time of incubation of the phospholipases A2 with platelet rich plasma. Parabromophenacyl bromide modified PLA2 enzymes lost their enzymatic activity as well as platelet aggregation inhibition activity suggesting the involvement of catalytic function in platelet aggregation inhibitory activity.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::c69fd59cfb73c991f5f4fadf370d42e3 https://pubmed.ncbi.nlm.nih.gov/11354251 Zobrazit plný text záznamu Full text from SpringerLink