| Autor: |
I, Rey, F, Schweighoffer, I, Barlat, J, Camonis, E, Boy-Marcotte, R, Guilbaud, M, Jacquet, B, Tocque |
| Rok vydání: |
1991 |
| Předmět: |
|
| Zdroj: |
Oncogene. 6(2) |
| ISSN: |
0950-9232 |
| Popis: |
The evidence presented here indicates that the domain containing the COOH-terminal part of the Saccharomyces cerevisiae SCD25 gene product (C-domain), which is homologous to the COOH-terminal part of CDC25 protein, can elicit activation of mammalian ras proteins in CHO cells. Transfection of expression vectors carrying the C-domain of SCD25, but not of CDC25, promotes the GTP-bound form of ras proteins as determined by analysis of the guanine nucleotides bound to ras proteins immunoprecipitated by Y13-259 mAb, and enhances transcription of a HIV-LTR-CAT construct. This is the first demonstration of the activation of ras proteins by transfection of a single heterologous gene. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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