| Autor: |
G E, Jørgensen, J I, Johnsen, F, Ponthan, P, Kogner, T, Flaegstad, T, Traavik |
| Rok vydání: |
2000 |
| Předmět: |
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| Zdroj: |
Medical and pediatric oncology. 35(6) |
| ISSN: |
0098-1532 |
| Popis: |
We reported previously that nearly all human neuroblastomas analyzed contain and express genomic DNA sequences deriving from the human polyomavirus BK (BKV) [Flaegstad et al.: Cancer Res 59:1160-1163, 1999].Here we show that the BKV large T antigen is expressed and bound to p53 in neuroblastoma cells and that this interference compromises the tumor suppressor function of p53.Treatment of neuroblastoma cells with large T antigen antisense constructs relocated active p53 to the nucleus. The relocation event was accompanied by enhanced p21(waf1/cip1) expression as well as induced apoptosis.Continuous antisense oligonucleotide treatment of nude rats with human neuroblastoma xenografts resulted in a significant but incomplete reduction of tumor growth compared to rats treated with saline. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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