Autor: |
S K, Raj, R M, Leiser, L, Plobner |
Rok vydání: |
1994 |
Předmět: |
|
Zdroj: |
Indian journal of experimental biology. 32(5) |
ISSN: |
0019-5189 |
Popis: |
RNA isolation from purified cucumber mosaic virus, CMV-U strain and cDNA synthesis was carried out. The coat protein gene (RNA4) region was amplified selectively by polymerase chain reaction (PCR) with CMV RNA4-specific primers. Double-stranded cDNA was cloned in PRT103 vector at Xho1/Kpn1 site and about 1kb insert obtained. The insert was partly sequenced which showed 50% sequence homology with CMV-Q, C and WL strains. |
Databáze: |
OpenAIRE |
Externí odkaz: |
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