Reactivation of glyceraldehyde-3-phosphate dehydrogenase using conjugates of monoclonal antibodies with polyelectrolyte complexes. An attempt to make an artificial chaperone

Autor:	M B, Dainiak, V A, Izumrudov, V I, Muronetz, Galaev IYu, B, Mattiasson
Rok vydání:	1999
Předmět:	Models Molecular Electrolytes Mice Protein Folding Enzyme Reactivators Protein Conformation Animals Antibodies Monoclonal Glyceraldehyde-3-Phosphate Dehydrogenases Rabbits In Vitro Techniques Dimerization Molecular Chaperones
Zdroj:	Journal of molecular recognition : JMR. 11(1-6)
ISSN:	0952-3499
Popis:	The simplified model of chaperone action when the inactive misfolded forms are removed from the reaction media preventing aggregation was developed using antibodies in combination with polyelectrolyte complexes. The antibodies, which bind specifically inactive dimers of glyceraldehyde-3-phosphate dehydrogenase but not native tetramers, were coupled covalently to poly(methacrylic acid). The treatment of inactivated GAPDH with this conjugate followed by its precipitation after equimolar addition of polycation, poly-(N-ethyl-4-vinylpyridinium bromide), resulted in a significant increase in the specific activity of the enzyme.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::c02f8301142d776e499e0c999799077e https://pubmed.ncbi.nlm.nih.gov/10076801 Zobrazit plný text záznamu Plný text