Involvement of stem cell factor and c-kit in corneal wound healing in mice
| Autor: | Miyamoto, Kazuhisa, Kobayashi, Takeshi, Hayashi, Yasuhito, Zhang, Yuan, Hara, Yuko, Higashine, Masakatsu, Shiraishi, Atsushi, Ohashi, Yuichi |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Collagen Type IV
Stem Cell Factor Wound Healing Epithelium Corneal Epithelial Cells Mice Transgenic Genistein Antibodies Fibronectins Mice Proto-Oncogene Proteins c-kit Bromodeoxyuridine Cell Adhesion Microscopy Electron Scanning Animals Laminin Oligopeptides Protein Kinase Inhibitors Research Article |
| Zdroj: | Molecular Vision |
| ISSN: | 1090-0535 |
| Popis: | Purpose To study the roles played by stem cell factor (SCF) and SCF receptor c-kit in wound healing of corneal epithelial cells. Methods A 2 mm corneal epithelial wound was made in control (WBB6F1+/+), SCF (Sl/Sld)-, and c-kit (W/Wv) mutant mice, and the speed of wound healing, 5-bromo-2’-deoxyuridine (BrdU) incorporation, and scanning electron microscopic (SEM) morphology of the corneas were examined. The incorporation of BrdU and the degree of cell attachment in cultured mouse corneal epithelial cells (MCECs) isolated from WBB6F1+/+, Sl/Sld, and W/Wv mice were examined. Cultured immortalized human corneal epithelial cells (HCECs) were examined by a cell attachment assay after their exposure to anti-SCF antibodies, tyrosine kinase inhibitor (genistein), and competitive Arg-Gly-Asp (RGD) peptide, as well as on cultures treated with extracellular matrix. Results The speed of corneal wound healing was slower in Sl/Sld and W/Wv mice than in controls (p |
| Databáze: | OpenAIRE |
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