Genome-Wide Profiling of Cap-Independent Translation Enhancing Elements in the Human Genome

Autor:	Wellensiek, Brian P., Larsen, Andrew C., Stephens, Bret, Kukurba, Kim, Waern, Karl, Briones, Natalia, Liu, Li, Snyder, Michael, Jacobs, Bertram L., Kumar, Sudhir, Chaput, John C.
Jazyk:	angličtina
Rok vydání:	2013
Předmět:	Genome Human Protein Biosynthesis Human Genome mRNA Display High-Throughput Nucleotide Sequencing Humans RNA Messenger 5' Untranslated Regions Peptide Chain Initiation Translational Internal Ribosomal Entry Sites (IRES) Article Gene Library HeLa Cells
Zdroj:	Nature methods
ISSN:	1548-7105 1548-7091
Popis:	We report an in vitro selection strategy to identify RNA sequences that mediate cap-independent initiation of translation. This method entails mRNA display of trillions of genomic fragments, selection for initiation of translation and high-throughput deep sequencing. We identified12,000 translation-enhancing elements (TEEs) in the human genome, generated a high-resolution map of human TEE-bearing regions (TBRs), and validated the function of a subset of sequences in vitro and in cultured cells.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::bb7507d307c2f96c4d0918a1a7daf6c7 http://europepmc.org/articles/PMC3731418 Zobrazit plný text záznamu Plný text ve formátu PDF