CD45RO expression on bovine T cells: relation to biological function
| Autor: | G P, Bembridge, N D, MacHugh, D, McKeever, E, Awino, P, Sopp, R A, Collins, K I, Gelder, C J, Howard |
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| Rok vydání: | 1995 |
| Předmět: |
CD4-Positive T-Lymphocytes
Base Sequence Interleukins Molecular Sequence Data hemic and immune systems chemical and pharmacologic phenomena CD8-Positive T-Lymphocytes complex mixtures Precipitin Tests Interferon-gamma immune system diseases T-Lymphocyte Subsets hemic and lymphatic diseases Animals Leukocyte Common Antigens Cattle Electrophoresis Polyacrylamide Gel Cell Division Research Article |
| Zdroj: | Immunology. 86(4) |
| ISSN: | 0019-2805 |
| Popis: | The 180,000 MW isoform of CD45 (CD45RO) has been identified in cattle with a novel monoclonal antibody (mAb) (IL-A116). This has allowed a more precise analysis of T-cell function in relation to CD45 isoform expression. Within the CD4+ and CD8+ T-cell populations, CD45RO+ and CD45RO- subsets were evident. Most CD4+ and CD8+ T cells that expressed the CD45RO isoform did not express the 220,000 and 205,000 MW isoforms recognized by mAb CC76. In contrast, the WC1+, CD2-, CD4-, CD8-, gamma delta T-cell receptor (TCR)+ T cells in bovine peripheral blood mononuclear cells (PBMC) were all CD45RO+. Monocytes and granulocytes were CD45RO+ but B cells were CD45RO-. Sorting experiments with CD4+ T cells from an immunized calf demonstrated that proliferative responses to ovalbumin (OVA) were entirely within the CD45RO+ subset. Following stimulation with concanavalin A (Con A) the CD45RO- subset of CD4+ T cells produced transcripts for interleukin-2 (IL-2) but not IL-4 or interferon-gamma (IFN-gamma), while the CD45RO+ subset produced mRNA for IL-2, IL-4 and IFN-gamma. Biologically active IL-2 was present in supernatants from both CD45RO+ and CD45RO-, CD4+ T cells, and IFN-gamma protein was identified by ELISA in supernatants from the CD45RO+ subset, confirming the production of cytokines implied by polymerase chain reaction (PCR). In contrast, sorting experiments with CD8+ T cells from animals immune to the protozoan parasite Theileria parva revealed substantial numbers of cytotoxic T-lymphocyte precursors in both the CD45RO+ and CD45RO- subsets. Thus it appears that although all antigenically primed CD4+ T cells remain CD45RO+, and expression of this molecule consequently identifies memory cells within PBMC, antigenically primed CD8+ T cells down-regulate CD45RO expression after activation. |
| Databáze: | OpenAIRE |
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