| Autor: |
S, Keay, C O, Zhang, A L, Trifillis, M K, Hise, J R, Hebel, S C, Jacobs, J W, Warren |
| Rok vydání: |
1996 |
| Předmět: |
|
| Zdroj: |
The Journal of urology. 156(6) |
| ISSN: |
0022-5347 |
| Popis: |
Interstitial cystitis (IC) is a chronic bladder disease of unknown etiology. We sought to determine whether a cytotoxin is present in the urine of IC patients that could cause the epithelial damage seen in this disease.Evidence for a cytotoxin was sought using both a neutral red uptake viability assay in T24 bladder epithelial cells, and a 3H-thymidine incorporation assay in primary normal adult bladder epithelial cells and FHS 738 Bl human fetal bladder cells.The neutral red assay in T24 cells indicated the presence of a cytotoxin in 2 of 9 IC patient urine specimens. However, the more sensitive assay of cell proliferation (3H-thymidine incorporation) in normal adult human bladder epithelial cells revealed antiproliferative activity in urine from 10 of 13 (77%) IC patients vs. 3 of 19 (16%) controls (two-way analysis of variance, p = .019). The antiproliferative activity of IC urine specimens was confirmed using FHS 738 Bl human fetal bladder cells. The antiproliferative urinary substance(s) appeared to be a low molecular weight (10,000 Da), heat stable, trypsin-sensitive factor(s).Because a denuded or damaged bladder epithelium is a central finding in IC, it is possible that the antiproliferative protein(s) contributes to the pathogenesis of this disease. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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