| Autor: |
An, Martens, Sofie, Ordies, Bart M, Vanaudenaerde, Stijn E, Verleden, Robin, Vos, Geert M, Verleden, Eric K, Verbeken, Dirk E, Van Raemdonck, Sandra, Claes, Dominique, Schols, Matthieu, Chalopin, Ira, Katz, Geraldine, Farjot, Arne P, Neyrinck |
| Rok vydání: |
2017 |
| Předmět: |
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| Zdroj: |
Medical Gas Research |
| ISSN: |
2045-9912 |
| Popis: |
Argon (Ar) is a noble gas with known organoprotective effects in rodents and in vitro models. In a previous study we failed to find a postconditioning effect of Ar during ex vivo lung perfusion (EVLP) on warm-ischemic injury in a porcine model. In this study, we further investigated a prolonged exposure to Ar to decrease cold ischemia-reperfusion injury after lung transplantation in a porcine model with EVLP assessment. Domestic pigs (n = 6/group) were pre-conditioned for 6 hours with 21% O2 and 79% N2 (CONTR) or 79% Ar (ARG). Subsequently, lungs were cold flushed and stored inflated on ice for 18 hours inflated with the same gas mixtures. Next, lungs were perfused for 4 hours on EVLP (acellular) while ventilated with 12% O2 and 88% N2 (CONTR group) or 88% Ar (ARG group). The perfusate was saturated with the same gas mixture but with the addition of CO2 to an end-tidal CO2 of 35-45 mmHg. The saturated perfusate was drained and lungs were perfused with whole blood for an additional 2 hours on EVLP. Evaluation at the end of EVLP did not show significant effects on physiologic parameters by prolonged exposure to Ar. Also wet-to-dry weight ratio did not improve in the ARG group. Although in other organ systems protective effects of Ar have been shown, we did not detect beneficial effects of a high concentration of Ar on cold pulmonary ischemia-reperfusion injury in a porcine lung model after prolonged exposure to Ar in this porcine model with EVLP assessment. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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