| Autor: |
Li-Li, Zhang, Aronson I, Arthur, Zi-Niu, Yu |
| Rok vydání: |
2005 |
| Zdroj: |
Yi chuan = Hereditas. 24(3) |
| ISSN: |
0253-9772 |
| Popis: |
In order to investigate the influence of the carbon source on the expression of cry1D -lacZ in Bacillus thuringiensis with different genetic backgrounds, cry1D -lacZ was introduced into different strains and cultured in liquid medium containing different carbon sources. Analysis of activity of beta-galactosidase showed that cry1D -lacZ expressed differently in the same host because of different carbon sources and differently in different hosts utilizing same carbon sources,suggesting that metabolism pathway of carbon source in HD-133-5 was special. Sodium succinate as carbon source could lead to earlier sporulation and earlier expression of cry1D -lacZ. When sodium acetate acted as carbon source, cry1D -lacZ could keep high, stable expression in different hosts. These results are helpful for future fermentation of Bacillus thuringiensis. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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