The RNA-binding protein Hfq assembles into foci-like structures in nitrogen starved
| Autor: | Josh, McQuail, Amy, Switzer, Lynn, Burchell, Sivaramesh, Wigneshweraraj |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
photoactivatable localization microscopy (PALM)
Nitrogen Escherichia coli Proteins RNA chaperone Escherichia coli (E. coli) small regulatory RNA (sRNA) Gene Expression Regulation Bacterial stress response Host Factor 1 Protein Adaptation Physiological Microbiology T7 phage bacteriophage Multiprotein Complexes Hfq protein Escherichia coli gene expression nitrogen starvation environmental adaptation gene regulation |
| Zdroj: | The Journal of Biological Chemistry |
| ISSN: | 1083-351X |
| Popis: | The initial adaptive responses to nutrient depletion in bacteria often occur at the level of gene expression. Hfq is an RNA-binding protein present in diverse bacterial lineages that contributes to many different aspects of RNA metabolism during gene expression. Using photoactivated localization microscopy and single-molecule tracking, we demonstrate that Hfq forms a distinct and reversible focus-like structure in Escherichia coli specifically experiencing long-term nitrogen starvation. Using the ability of T7 phage to replicate in nitrogen-starved bacteria as a biological probe of E. coli cell function during nitrogen starvation, we demonstrate that Hfq foci have a role in the adaptive response of E. coli to long-term nitrogen starvation. We further show that Hfq foci formation does not depend on gene expression once nitrogen starvation has set in and occurs indepen-dently of the transcription factor N-regulatory protein C, which activates the initial adaptive response to N starvation in E. coli. These results serve as a paradigm to demonstrate that bacterial adaptation to long-term nutrient starvation can be spatiotemporally coordinated and can occur independently of de novo gene expression during starvation. |
| Databáze: | OpenAIRE |
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