Fast nucleic acid amplification for integration in point-of-care applications

Autor:	Sabine, Brunklaus, Thomas E, Hansen-Hagge, Julia, Erwes, Julian, Höth, Mathieu, Jung, Daniel, Latta, Xenia, Strobach, Christian, Winkler, Marion, Ritzi-Lehnert, Klaus S, Drese
Rok vydání:	2012
Předmět:	Male Point-of-Care Systems Temperature Humans Reproducibility of Results Computer Simulation DNA Equipment Design Microfluidic Analytical Techniques Polymerase Chain Reaction Actins
Zdroj:	Electrophoresis. 33(21)
ISSN:	1522-2683
Popis:	An ultrafast microfluidic PCR module (30 PCR cycles in 6 min) based on the oscillating fluid plug concept was developed. A robust amplification of native genomic DNA from whole blood samples could be achieved at operational conditions established from systematic investigations of key parameters including heat transfer and in particular flow velocities. Experimental data were augmented with results from computational fluid dynamics simulations. The reproducibility of the current system was substantially improved compared to previous concepts by integration of a closed reservoir instead of utilizing a vented channel end at ambient pressure rendering the devised module suitable for integration into complex sample-to-answer analysis platforms such as point-of-care applications.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::ac92f03beac186b291cd99324bec45e8 https://pubmed.ncbi.nlm.nih.gov/23065712 Zobrazit plný text záznamu Plný text