[Transfection of bacterial cells by DNA of phage M13 entrapped in phospholipid vesicles]

Autor: N D, Beliaev, V G, Budker, T G, Maksimova, L P, Naumova, A V, Sokolov
Rok vydání: 1982
Předmět:
Zdroj: Molekuliarnaia biologiia. 16(3)
ISSN: 0026-8984
Popis: The possibility of transfection of bacterial cells by phage M13 DNA entrapped into phospholipid vesicles (liposomes) has been studied. Two types of liposomes differing in size were used. Entrapped DNA was transferred by liposomes into Ca2+-treated E. coli cells. Efficiency of the transfection in the case of small (ca. 400 A) liposomes was 2--3 orders of magnitude higher than that of free DNA extracted from such liposomes.
Databáze: OpenAIRE