An ABCG Transporter Functions in Rab Localization and Lysosome-Related Organelle Biogenesis in
| Autor: | Laura, Voss, Olivia K, Foster, Logan, Harper, Caitlin, Morris, Sierra, Lavoy, James N, Brandt, Kimberly, Peloza, Simran, Handa, Amanda, Maxfield, Marie, Harp, Brian, King, Victoria, Eichten, Fiona M, Rambo, Greg J, Hermann |
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| Rok vydání: | 2019 |
| Předmět: |
Organelle Biogenesis
Vesicular Transport Proteins Membrane Transport Proteins ATP Binding Cassette Transporter Subfamily G Endosomes Investigations Cytoplasmic Granules Protein Transport Phenotype rab GTP-Binding Proteins Mutation ATP Binding Cassette Transporter Subfamily G Member 2 Animals ATP-Binding Cassette Transporters Caenorhabditis elegans Caenorhabditis elegans Proteins Lysosomes |
| Zdroj: | Genetics |
| ISSN: | 1943-2631 |
| Popis: | ABC transporters couple ATP hydrolysis to the transport of substrates across cellular membranes. This protein superfamily has diverse activities resulting from differences in their cargo and subcellular localization. Our work investigates the role of the ABCG family member WHT-2 in the biogenesis of gut granules, a Caenorhabditis elegans lysosome-related organelle. In addition to being required for the accumulation of birefringent material within gut granules, WHT-2 is necessary for the localization of gut granule proteins when trafficking pathways to this organelle are partially disrupted. The role of WHT-2 in gut granule protein targeting is likely linked to its function in Rab GTPase localization. We show that WHT-2 promotes the gut granule association of the Rab32 family member GLO-1 and the endolysosomal RAB-7, identifying a novel function for an ABC transporter. WHT-2 localizes to gut granules where it could play a direct role in controlling Rab localization. Loss of CCZ-1 and GLO-3, which likely function as a guanine nucleotide exchange factor (GEF) for GLO-1, lead to similar disruption of GLO-1 localization. We show that CCZ-1, like GLO-3, is localized to gut granules. WHT-2 does not direct the gut granule association of the GLO-1 GEF and our results point to WHT-2 functioning differently than GLO-3 and CCZ-1. Point mutations in WHT-2 that inhibit its transport activity, but not its subcellular localization, lead to the loss of GLO-1 from gut granules, while other WHT-2 activities are not completely disrupted, suggesting that WHT-2 functions in organelle biogenesis through transport-dependent and transport-independent activities. |
| Databáze: | OpenAIRE |
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