Use of a batch-stirred reactor to rationally tailor biocatalytic polytransesterification

Autor:	B J, Kline, S S, Lele, P J, Lenart, E J, Beckman, A J, Russell
Rok vydání:	2000
Předmět:	Molecular Weight Bioreactors Polyesters Lipase Butylene Glycols Enzymes Immobilized Catalysis Biotechnology
Zdroj:	Biotechnology and bioengineering. 67(4)
ISSN:	0006-3592
Popis:	Despite favorable thermodynamics, high-molecular weight and low-dispersity polyesters are difficult to synthesize biocatalytically in organic solvents. We have reported previously that the elimination of solvent can improve the kinetics and apparent equilibrium significantly (Chaudhary et al., 1997a). We now present the design and use of a batch-stirred enzyme reactor to control the biocatalytic polymerization. Using the reactor, polyester having a molecular weight of 23,400 Da and a polydispersity of 1.69 was synthesized in only 1 h at 60 degrees C. Additional factors like enzyme-deactivation kinetics, enzyme specificity, and initial exothermicity were investigated to develop a better understanding of this complex reaction system.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::a881c2b86f23606616d70be503965c4d https://pubmed.ncbi.nlm.nih.gov/10620758 Zobrazit plný text záznamu Plný text