[The adaptation of an isolate of the hepatitis A virus to reproduction in cell cultures]

Autor:	V R, Farashian, A B, Bychenko, I A, Kuzaeva, E K, Artemov, Iu P, Kadoshnikov
Rok vydání:	1991
Předmět:	Virus Cultivation Nucleic Acid Hybridization Hepatitis A Embryo Mammalian Virus Replication Adaptation Physiological Macaca mulatta Cell Line Microscopy Electron Viral Proteins Chlorocebus aethiops DNA Viral Animals Humans Electrophoresis Polyacrylamide Gel Hepatovirus Cells Cultured
Zdroj:	Voprosy virusologii. 36(2)
ISSN:	0507-4088
Popis:	A HAV strain derived from a patient in Moscow multiplied in a continuous cell line PLC/PRF/5 at 32 degrees C (variant MI-1) and at 37 degrees C (variant MI-1.1). These two variants of the strain MI retained the ability for reproduction both at 32 degrees C and 37 degrees C after passages in cell culture. The strain MI also replicates in MK cells. Negative results on HAV cultivation were obtained in HEF-240 and FRhK-4 cell cultures. The MI-1 and MI-1.1 variants are typical of hepatitis A viruses by their physicochemical properties, the results of ELISA, protein electrophoresis and dot hybridization tests.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::a8477adb9af2f763256735a609d9fdb8 https://pubmed.ncbi.nlm.nih.gov/1652866 Zobrazit plný text záznamu