| Popis: |
When staining intact erythrocytes with DSM no quenching of its fluorescence with hems in the membrane was observed. The positively charged fluorescent probe DSM is proposed for estimating a relative change of the charge in phospholipid membrane surface, in erythrocyte ghosts, and in the membranes of some non-hemolyzed erythrocytes. Charged ligands induced a change of the bound probe fluorescence corresponding to the changes in the value and symbol of the charge of the membrane surface. By means of DSM and negatively charged probe of ANS interaction between dimedrol and intact erythrocytes and their ghosts was studied. It has been stated that therewith the ANS binding with the erythrocyte membrane increases, while DSM binding decreases, which points to a decrease of efficient negative charge of the cell surface in the presence of dimedrol. On the basis of cytofluorometric data the binding constant of dimedrol with the membrane of intact erythrocytes was determined. |
