[The bulk of tRNA in the extracts of animal cells is not complexed with proteins]

Autor: A T, Al'zhanova, E K, Davydova, L P, Obchinnikov
Rok vydání: 1982
Předmět:
Zdroj: Biokhimiia (Moscow, Russia). 47(12)
ISSN: 0320-9725
Popis: The bulk of tRNA from cytoplasmic cell extracts of rat liver sedimented in the postribosomal zone appears to be in a free state and is not complexed with proteins. Evidence for this assumption may be derived from the following experimental findings: i) the tRNA of the extract is not adsorbed on nitrocellulose filters under conditions of ribonucleoprotein sorption; ii) after formaldehyde fixation and contrifugation to equilibrium in CsCl or Cs2SO4 density gradients the tRNA of the extract is distributed as free tRNA; iii) the tRNA of the extract does not differ considerably from the deproteinated tRNA in sedimentation velocity.
Databáze: OpenAIRE