| Popis: |
Sensitivity to T-2 toxin, deoxynivalenol (DOV), zearalenon (ZL) and aflatoxin (AT) B1 was comparatively studied in yeast strains Saccharomyces fragilis 25 D, Candida pseudotropicalis 44 nk and Saccharomyces lactis BKMY-459, sensitive to T-2 toxin, and strain Bacillus megaterium BKMB-44, sensitive to toxic metabolites of Aspergillus species of fungi. Minimum amounts of T-2 toxin inducing on Silufol plates sites of the yeast strain growth suppression, comprised 20-50 ng, maximum sensitivity was recorded in Saccharomyces lactis BKMY-459. Minimum content of DOV suppressing the yeast growth comprised 10-50 micrograms, and that of ZL 10-25 micrograms. Bacillus megaterium BKMB-44 culture proved to be highly sensitive to AT B1, 1 microgram of which suppressed the strain growth. This culture was resistant to T-2 toxin and DOV in doses of 1 microgram and 200 micrograms, respectively. ZL suppressed Bacillus megaterium growth when its minimum content in the plaque was 0.5 = 1 microgram. The authors have considered the possibility of using the microorganism strains studied for screening toxic grain lots and toxigenic fungus strains. |
