| Autor: |
Ruo-Ying, Huang, Mei, Fang, Ying-Shu, Luo, Heng-Chuan, Liu, Qiao-Rong, Lu, Guo-Qing, Wang |
| Rok vydání: |
2014 |
| Předmět: |
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| Zdroj: |
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition. 45(1) |
| ISSN: |
1672-173X |
| Popis: |
A rapid and effective method with ethidium monoazide bromide (EMA) in combination with PCR (EMA-PCR) was established to detect live Enterohemorrhagic Eschrichia Coli O157:H7.The rfbE gene was used as the target gene for PCR detection of Eschrichia Coli O157:H7 by utilizing its pure isolates after the treatment of EMA as the template. The EMA concentration and reaction time was optimized.The use of 10 microg/mL or less EMA did not inhibit the PCR amplification of DNA derived from viable bacteria. The PCR amplification of DNA derived from 2 x 10(7) CFU/mL dead cells can be inhibited by 0.5 microg/mL EMA. The sensitivity of the method was 2 x 10(4) CFU/mL. The results demonstrated that it could detect 1% live bacteria from a mixed bacterial population.EMA-PCR can effectively detect live bacteria of O157:H7, it could be a potential rapid detection method applied in public health emergent events. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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