Miniaturized multichannel electrospray ionization emitters on poly(dimethylsiloxane) microfluidic devices

Autor:	J S, Kim, D R, Knapp
Rok vydání:	2001
Předmět:	Spectrometry Mass Electrospray Ionization Miniaturization Adrenocorticotropic Hormone Microchemistry Humans Equipment Design Angiotensin I Angiotensin III Rheology Peptide Fragments
Zdroj:	Electrophoresis. 22(18)
ISSN:	0173-0835
Popis:	A multichannel electrospray ionization (ESI) emitter was fabricated as part of a poly(dimethylsiloxane) (PDMS) microfluidic device using a three-layer photoresist process which also produces a self-alignment system to make a bonding between the top and bottom PDMS parts. The prototype device (2 cm high x 5 cm wide x 5 cm long) had 16-channels (30 microm wide x 50 microm deep) with emitters of 1 mm length and 60 degrees point angle. The PDMS emitter tips enabled interfacing the device to ESI-mass spectrometry; a stable electrospray from the tips was performed with limits of detection under 1 microM for reference peptides (adrenocorticotropic hormone fragment 1-17, angiotensin I and III).
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::a439e180c15d89b0bcb200241eb5bf1c https://pubmed.ncbi.nlm.nih.gov/11700731 Zobrazit plný text záznamu Plný text