| Autor: |
A O, Tsittser, Iu V, Ezepchuk, N O, Nakisbekov, A V, Li, V L, Semiotrochev, Iu L, Kiselev |
| Rok vydání: |
1990 |
| Předmět: |
|
| Zdroj: |
Molekuliarnaia genetika, mikrobiologiia i virusologiia. (9) |
| ISSN: |
0208-0613 |
| Popis: |
A new method for isolation and purification of Vibrio cholerae non-01 cytolysin has been elaborated. It includes the steps of concentration by ammonium sulphate, ion exchange chromatography on DE-52-cellulose, gel filtration via ultragel AsA-44 and chromatography on Mono Q. Cytolysin is shown to be a 60 kD and pI 6.2 protein. It is not inactivated by thiol-restoring agents and induces the production of precipitating antibodies. In contrast to choleragen the protein cytolytically affects erythrocytes and cells of the line L-929. It also possesses the lethal and oedematous effect and demonstrates the enteropathogenic effect on nursing rabbits. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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