Isolation of

Autor: Katsunori, Murota, Keiko, Ishii, Yuji, Mekaru, Miho, Araki, Yuto, Suda, Hiroaki, Shirafuji, Daisuke, Kobayashi, Haruhiko, Isawa, Tohru, Yanase
Rok vydání: 2021
Předmět:
Zdroj: Vector borne and zoonotic diseases (Larchmont, N.Y.). 21(10)
ISSN: 1557-7759
Popis: The circulation of arboviruses in livestock ruminants has often gone unrecognized owing to the fact that a significant percentage of arboviruses probably induce subclinical infections and/or negligible symptoms in infected animals. To determine the current situation of arbovirus circulation in the Yaeyama Islands, attempts to isolate viruses from bovine blood samples collected between 2014 and 2019 have been made. In total, 308 blood samples were collected during the study period, and 43 of them induced cytopathic effects (CPEs) in cell cultures. The identification of the CPE agents was performed by reported RT-PCR assays and a high-throughput analysis with a next-generation sequencing platform. The obtained viruses consisted of an orthobunyavirus (Peaton virus)
Databáze: OpenAIRE