Inactivation of ku80 in the mushroom-forming fungus Schizophyllum commune increases the relative incidence of homologous recombination

Autor:	Jan F, de Jong, Robin A, Ohm, Charissa, de Bekker, Han A B, Wösten, Luis G, Lugones
Rok vydání:	2010
Předmět:	DNA-Binding Proteins Fungal Proteins Genetics Microbial Recombination Genetic Gene Knockout Techniques Transformation Genetic Antigens Nuclear Schizophyllum Ku Autoantigen
Zdroj:	FEMS microbiology letters. 310(1)
ISSN:	1574-6968
Popis:	Schizophyllum commune is the only mushroom-forming fungus in which targeted gene deletions by homologous recombination have been reported. However, these deletions occur with a low frequency. To overcome this, the ku80 gene of S. commune was deleted. This gene is involved in the nonhomologous end-joining system for DNA repair. The Deltaku80 strain was not affected in growth and development. However, the transformation efficiency was reduced up to 100-fold. This was accompanied by a strong increase in the relative number of transformants with a homologous integration of a knockout construct. Genes sc15, jmj3 and pri2 were deleted in the Deltaku80 strain. In total, seven out of 10 transformants showed a gene deletion. This frequency will facilitate a systematic analysis of gene function in S. commune.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::9e1272357561844e8276fe67ce6a0773 https://pubmed.ncbi.nlm.nih.gov/20662932 Zobrazit plný text záznamu Plný text