In vivo evidence for possible up-regulating roles of lysophosphatidic acid around fertilization in rats
| Autor: | TAKEDA, Tae, SHIRASAKA, Miki, SUGIYAMA, Makoto, TERASHIMA, Ryota, KAWAMINAMI, Mitsumori, KURUSU, Shiro |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
autotaxin
Male Ovulation cumulus-oocyte complex Cumulus Cells Full Paper Physiology Phosphoric Diester Hydrolases Oviducts Immunohistochemistry Up-Regulation Phospholipases A2 fertilization Oocytes Animals lipids (amino acids peptides and proteins) rat Female biological phenomena cell phenomena and immunity Lysophospholipids Rats Wistar Receptors Lysophosphatidic Acid lysophosphatidic acid |
| Zdroj: | The Journal of Veterinary Medical Science |
| ISSN: | 1347-7439 0916-7250 |
| Popis: | Lysophosphatidic acid (LPA) produced by autotaxin (ATX) is recognized as a multi-functional mediator in mammalian reproduction. This study focused on possible effect(s) of LPA on ovulated cumulus-oocyte complexes (COCs) around fertilization in rats in vivo. Immunohistochemistry revealed the cell-type-dependent localization of candidates of synthetic enzymes, ATX and two phospholipases A2 isofroms, and LPA receptors LPA1−4 in ovulated COCs and in oviductal epithelium. The eggs ovulated with a form of COCs became denuded of cumulus cells and underwent fragmentation in the absence of fertilization. In vivo experiments of local administration in non-copulated rats demonstrated that eggs denudation was increased by LPA and decreased by anti-ATX antibody and that fragmentation was inhibited by LPA and stimulated by an ATX chemical inhibitor. Furthermore, LPA administration in adult copulated rats increased the rate of cleaved embryos significantly. Obtained results suggest the presence of LPA synthesis and action system in ovulated COCs within the oviductal ampulla and positive actions of LPA possibly at multiple sites around fertilization in rats. |
| Databáze: | OpenAIRE |
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