Cyclooxygenase-2 expression in human adenocarcinoma cell line HT29 cl.19A
| Autor: | S, Battu, H, Chable-Rabinovitch, M, Rigaud, J L, Beneytout |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Arachidonic Acid
Membrane Proteins Immunohistochemistry Polymerase Chain Reaction Culture Media Serum-Free Recombinant Proteins Culture Media Isoenzymes Cyclooxygenase 2 Prostaglandin-Endoperoxide Synthases Animals Humans Cattle Cloning Molecular Colorectal Neoplasms Promoter Regions Genetic HT29 Cells Cell Division DNA Primers |
| Zdroj: | Anticancer research. 18(4A) |
| ISSN: | 0250-7005 |
| Popis: | Overexpression of cyclooxygenase-2 (COX-2) has been implicated in carcinogenesis of human colorectal cancer which is one of the leading types of cancer in Western countries.We analyzed the COX-2 expression and activity using RT-PCR, Western blot, immunocytochemistry, RP-HPLC and EIA analysis in 0% and 10% fetal bovine serum (FBS) cultured cells.HT 29 cl.19A cells exhibited a COX-2 expression called "constitutive" in the absence of FBS in culture media. This particular expression was not the result of a mutation of the HT29 cl.19A COX-2 gene promotor.In our study, the human colon adenocarcinoma cell line, HT29 cl.19A, expressed COX-2 abnormally. This expression appeared to be at the same time inducible by the action of classical exogenous inducers such as FBS or interleukin-1 beta and "constitutive" if none of these compounds were present. |
| Databáze: | OpenAIRE |
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