| Autor: |
Alexandre Dias Tavares, Costa, Steffanie Skau, Amadei, Amanda, Bertão-Santos, Tuany, Rodrigues |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Journal of visualized experiments : JoVE. (179) |
| ISSN: |
1940-087X |
| Popis: |
Real-time PCR (qPCR) is a remarkably sensitive and precise technique that allows for amplifying minute amounts of nucleic acid targets from a multitude of samples. It has been extensively used in many research areas and achieved industrial application in fields such as human diagnostics and trait selection in crops of genetically modified organisms (GMO) crops. However, qPCR is not an error-proof technique. Mixing all reagents into a single master mix subsequently distributed onto 96 wells of a regular qPCR plate might lead to operator mistakes such as incorrect mixing of reagents or inaccurate dispensing into the wells. Here, a technique called gelification is presented, whereby most of the water present in the master mix is substituted by reagents that form a sol-gel mixture when submitted to a vacuum. As a result, qPCR reagents are effectively preserved for a few weeks at room temperature or a few months at 2-8 |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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