| Autor: |
Meng-Die, Qi, Jian, Wang, Xiao-Jing, Ma, Quan, Zhang, Fang-Fang, Wang, Ying, Kang, Hui-Xin, Lin, Yong, Liu |
| Rok vydání: |
2017 |
| Předmět: |
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| Zdroj: |
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. 43(2) |
| ISSN: |
1001-5302 |
| Popis: |
Andrographolide is a main active ingredient in traditional Chinese medicine Andrographis paniculata，with a variety of pharmacological activity，widely used in clinical practice. However its biosynthetic pathway has not been resolved. Cytochrome P450 reductase provides electrons for CYP450 and plays an important role in the CYP450 catalytic process. In this study，the coding sequence of A. paniculata CPR was screened and cloned by homologous alignment，named ApCPR4. The ApCPR4 protein was obtained by prokaryotic expression. After isolation and purification，the enzyme activity was identified |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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