| Popis: |
Depending on the dose of X-rays, in-vitro irradiation of Ehrlich-ascitic carcinoma cells of the mouse affected both respiration and glycolysis. 15,000 R irradiation suppressed the aerobic and anaerobic energy metabolism rather strongly followed by a reduction of the "take" and growth of the subcutaneously injected tumour cells, as opposed to the growth behaviour of non-irradiated cells. In analogy, tetanus mortality rates were reduced in the mouse tumour-tetanus assay with 15,000 R irradiated cells. On the other hand, irradiation with 2,000 R of Ehrlich carcinoma cells resulted in unchanged rates of respiration and glycolysis, in spite of the strongly limited growth capacity of the tumour cells. The tumour-tetanus assay of the mouse showed good correlation with subcutaneous tumour growth; no such correlation was found in the tetanus assay and the manometric values of respiration and glycolysis with 2,000 R irradiated tumour cells.--After subcutaneous injection of mixed cell suspensions consisting of 1 x 10(5) viable and 1 x 10(6) 15,000 R irradiated Ehrlich-ascitic carcinoma cells as well as of 3 x 10(2) tetanus spores per single dose, we observed similar rates of tumour growth, or tetanus mortality, respectively, if 1 x 10(5) viable tumour cells alone were administered together with 3 x 10(2) tetanus spores, without addition of irradiated tumour cells. |
