| Autor: |
Evelin, Horváth, Bernadett, Balla, János, Kósa, Péter András, Lakatos, Áron, Lazáry, Dániel, Németh, Hasan, Jozilan, Áron, Somorácz, Anna, Korompay, Benedek, Gyöngyösi, Katalin, Borka, András, Kiss, Péter, Kupcsulik, Zsuzsa, Schaff, Ferenc, Szalay |
| Rok vydání: |
2016 |
| Předmět: |
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| Zdroj: |
Orvosi hetilap. 157(48) |
| ISSN: |
0030-6002 |
| Popis: |
1,25-Dihydroxy vitamin DWe examined mRNA and protein expression differences in 1,25-Dihydroxy vitamin DSnap-frozen tissues from 13 patients were studied for mRNA and protein expression of CYP24A1. Paraffin-embedded tissues from 36 patients were used to study mRNA of VDR and CYP27B1. mRNA expression was measured by RT-PCR, CYP24A1 protein was detected by immunohistochemistry.Expression of VDR and CYP27B1 was significantly lower in hepatocellular carcinoma compared with non-tumorous liver (p0.05). The majority of the HCC samples expressed CYP24A1 mRNA, but neither of the non-tumorous liver. The gene activation was followed by CYP24A1 protein synthesis.The presence of CYP24A1 mRNA and the reduced expression of VDR and CYP27B1 mRNA in human hepatocellular carcinoma samples indicate decreased bioavailability of 1,25-Dihydroxy vitamin D |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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