[Assay of sulfhydryl groups in cardiac myofibrillar proteins: effect of oxygen radicals in vitro]

Autor:	G, Berson, A, Marquet
Jazyk:	francouzština
Rok vydání:	1990
Předmět:	Male Oxygen Xanthine Oxidase Free Radicals Myofibrils Purines Myocardium Animals Muscle Proteins Rats Inbred Strains Sulfhydryl Compounds In Vitro Techniques Rats
Zdroj:	Comptes rendus des seances de la Societe de biologie et de ses filiales. 184(1)
ISSN:	0037-9026
Popis:	Myofibrils from rat hearts were prepared in conditions maintaining their redox state, and their sulfhydryl groups were measured using a solution of urea and sodium dodecyl sulfate (SDS) as denaturant. The sulfhydryl content was 92 n mol/mg of protein, indicating that cysteins are in reduced form. In the presence of superoxide radicals generated in vitro with purine and xanthine oxidase, the myofibrillar sulfhydryl groups were oxidized.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::8efc6a67357ca8d5baf613c1183783cb https://pubmed.ncbi.nlm.nih.gov/2150778 Zobrazit plný text záznamu