Can proteomics elucidate mechanisms of antimicrobial resistance in
| Autor: | Jianhe, Peng, Julie, Russell, Sarah, Alexander |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Proteomics
Whole Genome Sequencing Lipoproteins Bacterial Toxins Glycosyltransferases Membrane Proteins Membrane Transport Proteins World Health Organization Neisseria gonorrhoeae Bacterial Proteins Tandem Mass Spectrometry Drug Resistance Multiple Bacterial Drug Resistance Bacterial Humans ATP-Binding Cassette Transporters Macrolides Bacterial Outer Membrane Proteins Chromatography Liquid Fluoroquinolones |
| Zdroj: | Sexually transmitted infections. 96(5) |
| ISSN: | 1472-3263 |
| Popis: | Antimicrobial resistance (AMR) in14 WHO reference strains were propagated, pooled stable isotope labelled lysates were used as an internal standard (IS). Protein lysates were mixed with IS, digested with trypsin and fractionated before analysis by nano-LC/MS/MS, in triplicate. The susceptible strain WHO F was used as reference to which the proteomic profiles of other strains were compared. Hierarchical clustering and permutation adjusted t-tests were performed to find proteins with significant fold changes.Standardised, reproducible protein expression profiles inA robust method was developed to study protein expression in |
| Databáze: | OpenAIRE |
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