Oxidative Stress Promotes Doxorubicin-Induced Pgp and BCRP Expression in Colon Cancer Cells Under Hypoxic Conditions
| Autor: | Martha L, Pinzón-Daza, Yenith, Cuellar-Saenz, Francisco, Nualart, Alejandro, Ondo-Mendez, Lilia, Del Riesgo, Fabio, Castillo-Rivera, Ruth, Garzón |
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| Rok vydání: | 2016 |
| Předmět: |
Microscopy
Confocal Cell Survival Enzyme-Linked Immunosorbent Assay Hypoxia-Inducible Factor 1 alpha Subunit Cell Hypoxia Neoplasm Proteins Oxidative Stress Doxorubicin Cell Line Tumor Colonic Neoplasms DNA-(Apurinic or Apyrimidinic Site) Lyase ATP Binding Cassette Transporter Subfamily G Member 2 Humans ATP Binding Cassette Transporter Subfamily B Member 1 Reactive Oxygen Species HT29 Cells |
| Zdroj: | Journal of cellular biochemistry. 118(7) |
| ISSN: | 1097-4644 |
| Popis: | P-glycoprotein (Pgp) and breast cancer resistance protein (BCRP) are ATP binding cassette (ABC) transporters that are overexpressed in different drug-resistant cancer cell lines. In this study, we investigated whether doxorubicin promotes Pgp and/or BCRP expression to induce drug resistance in colon cancer cells under hypoxic conditions. We analyzed HIF-1α activity via ELISA, Pgp, and BCRP expression by qRT-PCR and the relationship between doxorubicin uptake and ABC transporter expression via confocal microscopy in HT-29WT and HT-29 doxorubicin-resistant colon cancer cells (HT-29DxR). These cells were treated with doxorubicin and/or CoCl |
| Databáze: | OpenAIRE |
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