Dioscin Derived from Solanum melongena L. 'Usukawamarunasu' Attenuates α-MSH-Induced Melanogenesis in B16 Murine Melanoma Cells via Downregulation of Phospho-CREB and MITF
| Autor: | Atsuyoshi, Nishina, Kodai, Ebina, Motohiko, Ukiya, Makoto, Fukatsu, Mamoru, Koketsu, Masayuki, Ninomiya, Daisuke, Sato, Hirokazu, Kimura |
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| Rok vydání: | 2015 |
| Předmět: |
Melanins
Microphthalmia-Associated Transcription Factor Membrane Glycoproteins Monophenol Monooxygenase Plant Extracts Melanoma Experimental Down-Regulation Diosgenin Intramolecular Oxidoreductases Mice alpha-MSH Animals Solanum melongena Phosphorylation Cyclic AMP Response Element-Binding Protein Extracellular Signal-Regulated MAP Kinases Oxidoreductases |
| Zdroj: | Journal of food science. 80(10) |
| ISSN: | 1750-3841 |
| Popis: | This study aimed to chemically isolate and explore an antimelanogenesis inducer in extracts of Solanum melongena L. "Usukawamarunasu" eggplant. We successfully identified dioscin ([25R]-Spirost-5-en-3β-yl) 2-O-(6-deoxy-α-L-mannopyranosyl) - 4-O-(6-deoxy-α-L-mannopyranosyl)-β-D-glucopyranoside] in the plant, and examined the effects of α-melanocyte-stimulating hormone (MSH)-induced melanogenesis in B16 murine melanoma cells by this plant-derived dioscin. Immunoblot analysis suggested that dioscin reduced the expression of tyrosinase, tyrosinase-related protein-1 (TRP-1), and TRP-2, resulting in inhibition of intracellular production of melanin. In addition, dioscin caused reduction of phosphorylated cAMP-responsive element binding protein 1 transcription factors (CREB), which led to a reduction of microphthalmia-related transcription factor (MITF) in α-MSH-stimulated cells, but did not affect phosphorylation of extracellular signal-regulated kinase. Furthermore, dioscin significantly downregulated the expression of tyrosinase, TRP-1, and TRP-2, which led to the reduction of α-MSH-induced melanogenesis in B16 cells. These results suggest that dioscin may decrease the level of MITF via inhibition of phosphorylation of CREB in α-MSH-induced melanogenesis in B16 cells. |
| Databáze: | OpenAIRE |
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