| Autor: |
Wen-Te, Kao, Chun-Yu, Lin, Lung-Ta, Lee, Ping-Ping H, Lee, Chin-Chun, Hung, Yung-Sheng, Lin, Shih-Hsun, Chen, Ferng-Chun, Ke, Jiuan-Jiuan, Hwang, Ming-Ting, Lee |
| Rok vydání: |
2008 |
| Předmět: |
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| Zdroj: |
Anticancer research. 28(4B) |
| ISSN: |
0250-7005 |
| Popis: |
In this study, highly invasive tumor cell lines (designated A431-I, -II and -III) derived from parental A431 tumor cells (A431-P) were isolated by three successive passages through a Boyden chamber with matrigel-coated membrane support. The invasive potential and the activity of secreted MMP-9 of each sub-line increased significantly compared to the A431-P (A431-IIIA431-IIA431-I) as evidenced by the in vitro invasion assay, gelatin zymography and immunoblotting analyses. RT-PCR results also revealed the elevated expression of MMP-9 in A431-III. We further characterized the A431-III sub-line and found these cells exhibited a greater potential for attachment and spreading on fibronectin-coated substratum and for migration. The A431-III cells displayed multiple cytoplasmic extensions with focal contacts (vinculin-positive staining) during cell spreading within 30 min. We also noticed an increase in FAK phosphorylation, but no significant change in FAK protein level in the A431-III sub-line compared to those of A431-P cells. Together, these results demonstrate that the greater invasion potential exhibited by the highly invasive A431-III subline is likely attributed to an increased ability for attachment, spreading and migration, as well as increased MMP activity. Thus, A431-P and the highly invasive A431-III sub-line could be an excellent model for studying the mechanism of cancer metastasis. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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