| Autor: |
Szu-Cheng, Kuo, Chao-Yi, Teng, Yi-Jung, Ho, Ying-Ju, Chen, Tzong-Yuan, Wu |
| Rok vydání: |
2016 |
| Předmět: |
|
| Zdroj: |
Methods in molecular biology (Clifton, N.J.). 1426 |
| ISSN: |
1940-6029 |
| Popis: |
Chikungunya virus (CHIKV) is the etiologic agent of Chikungunya fever and has emerged in many countries over the past decade. There are no effective drugs for controlling the disease. A bicistronic baculovirus expression system was utilized to co-express CHIKV structural proteins C (capsid), E2 and E1 and the enhanced green fluorescence protein (EGFP) in Spodoptera frugiperda insect cells (Sf21). The EGFP-positive Sf21 cells fused with each other and with uninfected cells to form a syncytium is mediated by the CHIKV E1 allowing it to identify chemicals that can prevent syncytium formation. The compounds characterized by this method could be anti-CHIKV drugs. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
