Disruption and basic functional analysis of six novel ORFs of chromosome XV from Saccharomyces cerevisiae

Autor:	M J, Lafuente, C, Gancedo
Rok vydání:	1999
Předmět:	Antigens Fungal Genes Fungal Molecular Sequence Data Membrane Proteins Saccharomyces cerevisiae Polymerase Chain Reaction Fungal Proteins Open Reading Frames Phenotype Amino Acid Sequence Chromosomes Fungal Cloning Molecular Carrier Proteins Sequence Alignment Gene Deletion
Zdroj:	Yeast (Chichester, England). 15(10B)
ISSN:	0749-503X
Popis:	We report the disruption and functional analysis of six open reading frames (ORFs) from chromosome XV, namely YOL155c, YOL154w, YOL119c, YOL118c, YOR301w and YOR306c, in FY1679 and CEN.PK2 backgrounds. We constructed replacement cassettes and cloned each ORF into the pRS416 centromeric plasmid. No obvious phenotype was observed for the corresponding deleted strains with respect to growth, mating or sporulation. YOL155c encodes a protein with a secretion signal and putative GPI-anchor recognition site and is possibly a cell wall protein, although its deletion did not present morphogenetic defects under any of the conditions tested. Although YOL119c and YOR306c are members of the monocarboxylate permease family, the growth of the double disruptant in acetate, lactate and pyruvate was similar to that of the parental strains.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::805e747d96c9cb4397b8eeac1e6c12cf https://pubmed.ncbi.nlm.nih.gov/10407273 Zobrazit plný text záznamu Plný text