| Autor: |
J R, Barrio, R E, Keen, J R, Ropchan, N S, MacDonald, F J, Baumgartner, H C, Padgett, M E, Phelps |
| Rok vydání: |
1983 |
| Předmět: |
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| Zdroj: |
Journal of nuclear medicine : official publication, Society of Nuclear Medicine. 24(6) |
| ISSN: |
0161-5505 |
| Popis: |
L-[1-11C]leucine, suitable for the determination of cerebral protein synthesis rates in man using positron emission tomography, has been synthesized using a modified Bucherer-Strecker reaction sequence. The isolation of the pure L-amino acid isomer from the enantiomeric mixture, initially obtained using either an open or closed reaction vessel, was achieved using a D-amino acid oxidase/catalase enzyme complex immobilized on a Sepharose support. The O2 required by the D-amino acid oxidase as the hydrogen acceptor was supplied by catalase. The L-[1-11C]leucine was obtained with a radiochemical purity of greater than 99% and with a radiochemical yield of 25%. Using a remote, semiautomated synthesis system, typical production time was 30-40 min after preparation of H11CN. The use of immobilized enzymes for rapid and effective resolution of amino acid enantiomers eliminates the possibility of protein contamination and assures the production of a sterile, pyrogen-free product. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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