The effects of angiopoietin-1 and -2 on tumor growth and angiogenesis in human colon cancer
| Autor: | S A, Ahmad, W, Liu, Y D, Jung, F, Fan, M, Wilson, N, Reinmuth, R M, Shaheen, C D, Bucana, L M, Ellis |
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| Rok vydání: | 2001 |
| Předmět: |
Male
Membrane Glycoproteins Neovascularization Pathologic Transplantation Heterologous Mice Nude Proteins Apoptosis Blotting Northern Transfection Angiopoietin-2 Mice Proliferating Cell Nuclear Antigen Protein Biosynthesis Colonic Neoplasms Angiopoietin-1 Animals Humans Endothelium Vascular HT29 Cells Cell Division Neoplasm Transplantation |
| Zdroj: | Cancer research. 61(4) |
| ISSN: | 0008-5472 |
| Popis: | Angiopoietin (Ang) 1 and Ang-2 are important regulators of endothelial cell survival. Current models suggest that an increase in Ang-2 expression in endothelial cells leads to initiation of angiogenesis. We stably transfected HT29 colon cancer cells with cDNA constructs for Ang-1 or -2 or with vector alone, injected the cells s.c. into nude mice, and assessed tumor growth. Immunohistochemical analyses confirmed sustained increases of Ang-1 and -2 in the tumors. The tumors produced by the Ang-2-transfected cells were larger than the tumors produced in the other groups; those tumors also had higher vessel counts and proliferative indices than tumors in the other groups. Tumors produced by the Ang-1 transfectants had fewer vessels and lower tumor cell proliferative indices than tumors in the other groups. These data suggest that imbalances between Ang-1 and -2 that result in a net gain of Ang-2 activity lead to enhanced tumor angiogenesis and growth. |
| Databáze: | OpenAIRE |
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