Isolation, purification and characterization of naturally derived Crocetin beta-d-glucosyl ester from
| Autor: | Mudasir A, Mir, Shabir Ahmad, Ganai, Sheikh, Mansoor, Sumira, Jan, P, Mani, Khalid Z, Masoodi, Henna, Amin, Muneeb U, Rehman, Parvaiz, Ahmad |
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| Rok vydání: | 2019 |
| Předmět: |
Crocetin beta-d-glucosyl ester
DPPH 2 2-diphenyl-1-picrylhydrazyl FBS Fetal Bovine serum NMR Nuclear magneticresonance TLC Thin layer chromatography LC-MS/MS Liquid chromatography–mass spectrometry Article Saffron Breast cancer UV Ultra violet Molecular docking MTT 3-(4 5–dimethyl thiazol–2–yl)–5–diphenyltetrazolium bromide Floral biowastes Antioxidant DMEM Dulbecco’s Modified Eagle’s Medium FTIR Fourier-transform infrared spectroscopy |
| Zdroj: | Saudi Journal of Biological Sciences |
| ISSN: | 1319-562X |
| Popis: | Saffron plant (Crocus sativus L.) is being used as a source of saffron spice and medicine to cure or prevent different types of diseases including cancers. We report the isolation, characterization of bioactive small molecule ([crocetin (β-d-glucosyl) ester] from the leaf biowastes of saffron plant of Kashmir, India. MTTC assay and Bio-autography aided approach were used to assess anti-oxidant activity and anti-cancer properties of crocin (s) against DPPH free radical and breast cancer cell line respectively. Crocetin beta-d-glucosyl ester restrained proliferation of human breast adeno-carcinoma cell model (MCF-7) without significantly affecting normal cell line (L-6). Further studies involving molecular mechanics generalized born surface area and molecular docking showed that crocetin beta-d-glucosyl ester exhibits strong affinity for estrogen receptor alpha and histone deacetylase 2 (crucial receptors involved in breast cancer signalling) as evidenced by the negative docking score and binding free energy (BFE) values. Therefore, crocetin beta-d-glucosyl ester from Crocus sativus biowastes showed antiproliferative effect possibly by inhibiting estrogen receptor alpha and HDAC2 mediated signalling cascade. |
| Databáze: | OpenAIRE |
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